Ocean-optics OOIBase32 Manuale Utente

OOIBase32 Spectrometer Operating Software Installation Installation and Operation Manual Document Number 000-20000-020-02-0505 Office

About This Manual Chapter Description Chapter 10: Other Menu Functions Contains descriptions of options and functions available from other menus in

A: Experiment Tutorials 2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about 3500 counts. If ne

A: Experiment Tutorials Transmission Experiments Transmission is the percentage of energy passing through a sample relative to the amount that pa

A: Experiment Tutorials 2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about 3500 counts. If ne

A: Experiment Tutorials Reflection Experiments Reflection is the return of radiation by a surface, without a change in wavelength. Reflection can

A: Experiment Tutorials ► Procedure Perform the following steps to take reflection measurements using OOIBase32: 1. Place OOIBase32 in scope mode

A: Experiment Tutorials Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength emitted from a radiant sample. In r

A: Experiment Tutorials ► Procedure Perform the following steps to take a relative irradiance measurement using OOIBase32: 1. Place OOIBase32 in s

A: Experiment Tutorials 7. Click the Save icon on the toolbar or select File | Save | Processed from the menu bar to save the spectrum to disk. No

A: Experiment Tutorials f. Select a multiplicative factor to apply to the data before plotting or storing. Then, select an additive constant or off

A: Experiment Tutorials 3. Enter a value in the Write Data to Disk Every X Acquisitions box to set the frequency for data saves. OOIBase32 saves

Chapter 1 OOIBase32 Introduction Product Overview OOIBase32 Spectrometer Operating Software (OOIBase32) is Ocean Optics’ next generation of operating

A: Experiment Tutorials 8. Enter a value to set the Duration for the entire time acquisition process. Be sure to select Hours, Minutes, Seconds, or

Appendix B Toolbars Overview The following section contains descriptions of the options available from the various dockable toolbars in OOIBase32. In

B: Toolbars To enable or disable the display of the other various toolbars, right click in the gray area above the spectral window and below the men

B: Toolbars Platinum Functions These options are only available in the OOIBase32 Platinum version. Consult the help system of OOIBase32 Platinum fo

B: Toolbars The table below details the options available in the Acquisition Parameters toolbar: Option Description Integ. Time (msec) Specifies the

B: Toolbars The table below details the options available in the Acquisition Parameters toolbar: Icon Function Description Store Dark This optio

B: Toolbars Icon Function Description Cursor Big Right Moves the cursor 25 pixels to the right. Cursor Peak Right Moves the cursor to the next

B: Toolbars Icon Function Description Absorbance Mode This command switches the current window into Absorbance mode. Transmission Mode This co

B: Toolbars USB-LS-450 This toolbar contains shortcuts to options available from the Spectrum | Configure Data Acquisition menu in OOIBase32, as wel

B: Toolbars USB-ISS-UV/VIS This toolbar contains OOIBase32 options specialized for use with the USB-ISS-UV/VIS Integrated Sampling System. The tab

1: OOIBase32 Introduction With OOIBase32, you can perform time-acquisition experiments for kinetics applications. As part of the time-acquisition fu

B: Toolbars Icon Function Description Print Prints the currently displayed spectra. Help Opens the OOIBase32 help system. Store Global Dark T

B: Toolbars Icon Function Description Cursor Big Right Mo ves the cursor 25 pixels to the right. Cursor Peak Right Moves the cursor to the nex

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Appendix C File Formats Overview There are several types of files created by OOIBase32. You can choose to view and edit any of these tab-delimited AS

C: File Formats The following data is an example of a Spectral Data File: OOIBase32 Version 2.0.1.3 Data File ++++++++++++++++++++++++++++++++++++ D

C: File Formats The information provided in an Experimental Parameters File includes settings information such as the active display settings file

C: File Formats Reference Filename= Sample Filename= Sample Dark Filename= [Channel1] Enabled=0 Dark Stored=0 Reference Stored=0 Sample Dark Stored=

C: File Formats [Channel7] Enabled=0 Dark Stored=0 Reference Stored=0 Sample Dark Stored=0 Dark Filename= Reference Filename= Sample Filename= Sam

C: File Formats An example of a Display Properties File follows: [WindowPlacement] WindowPlacement=0,1,-1,-1,-4,-23,-6,-25,1020,656 [GraphSettings\

C: File Formats BackgroundColor=12632256 Border=1 BorderWidth=2 Orientation=1 FontName=Arial FontSize=16 OverlayShowFilename=1 Floating=1 ShowCurs

Chapter 2 Configuring OOIBase32 Overview The following sections will guide you in configuring your OOIBase32 software and your Ocean Optics hardware

C: File Formats ReferenceType Type of reference monitoring applied, 0=none, 1=single point, 2=wavelength-by-wavelength, 3=integrated intensity Refer

C: File Formats NLCoef7=0.000000e+000 ChannelEnabled=1 DisplayStart=500.000000 DisplayEnd=777.945801 DisplayStartPixel=429 DisplayEndPixel=1205 Re

C: File Formats Frequency=10 FrequencyUnit=Seconds Duration=11 DurationUnit=Seconds ContinueUntilManuallyStopped=0 ShowValuesInStatusBar=1 [ChannelA

C: File Formats Plotted=0 RateOnly=0 RateBandwidth=0 SpectrometerChannel=Master [ChannelF] Enabled=0 Wavelength=500.000000000000000 Pixel=429 Band

C: File Formats Grams/32 SPC Files OOIBase32 can save and open data files in the Galactic Software GRAMS/32® SPC file format. You can obtain details

Index . .display file, 27 .spec file, 27 .TimeParameters file, 27 A A/D Converter, 3 A/D Interface, 51 About OOIBase32, 85 Absorbance Experiments, 88

Index C (Cont'd) Create a new spectral window, 15 Cursor, 41 Configure, 41 Enable, 41 Enabled, 41 Cursor Icons, 105 Custom Mode, 72 D Dark, 16,

Index F (Cont'd) Processed, 17 Reference, 16 Sample, 16 Sample Dark, 16 Print, 20 Print Preview, 20 Print Setup, 20 Save, 17 Dark, 18 Experime

Index L Legend, 43 Configure, 44 Enable, 44 Show Overlay Filename, 45 light source drift, 52 Line Color, 32, 33 Line Style, 32, 33 Line Width, 32, 3

Index S (Cont'd) Scope Mode Minus Dark, 70 Scope Mode Saturation Threshold, 29 Script Menu, 83 Script-defined Custom Mode, 72 Second Coefficie

2: Configuring OOIBase32 Note When using a USB-based spectrometer (such as the USB2000 or HR2000), you do not need to perform this step. Proceed to

Index T (Cont'd) Start, 80 Stop, 81 Suspend Graph Display, 81 Time Acquisition Channel Configuration dialog box, 77 Time Acquisition Configurat

2: Configuring OOIBase32 4. Read the Read Me file and click the Next button. The Choose Destination Location screen appears. 5. Click the Browse

2: Configuring OOIBase32 6. Click the Yes button to back up replaced files (OOIBase32 prompts you for a backup location), or click the No button to

2: Configuring OOIBase32 8. Click the Next button to begin installation. The OOIBase32 Platinum password screen appears. 9. Enter your OOIBase32 P

2: Configuring OOIBase32 11. Click the OK button to restart your computer. You have now installed the OOIBase32 software. Configuring OOIBase32 an

2: Configuring OOIBase32 This dialog box prompts your to enter a user name and serial number, which OOIBase32 places in the header of certain data f

Copyright © 1999-2005 Ocean Optics, Inc. All rights reserved. No part of this publication may be reproduced,

2: Configuring OOIBase32 ► Procedure 1. Select your spectrometer type from the Spectrometer Type drop-down menu. 2. Select the appropriate A/D co

2: Configuring OOIBase32 ► Procedure 1. Select Spectrometer | Configure from the OOIBase32 menu. The Spectrometer Configuration screen appears. 2.

2: Configuring OOIBase32 5. Select the Detector Linearity tab and verify that OOIBase32 has loaded the intercept and coefficients from the .SPEC fi

2: Configuring OOIBase32 6. Click the OK button to save the data and close the Spectrometer Configuration screen. Upon exiting OOIBase32, the softwa

2: Configuring OOIBase32 Configure Sampling Optics Once you configure OOIBase32, you must configure the components in your sampling system. Due to t

Chapter 3 File Menu Functions Overview This chapter details the various options and functions available from the File menu in OOIBase32. Where applic

3: File Menu Functions 5. Create different parameters for the new spectral window, such as a different integration time. Remember that one spectra

3: File Menu Functions Processed - General Functions Toolbar Processed spectra are spectra taken while in scope, absorbance, transmission, or rela

3: File Menu Functions The following sections detail the types of data you can save: Dark A dark spectrum is a spectrum taken with the light path bl

3: File Menu Functions Autoincrement Filenames The Autoincrement Filenames option allows you to name and save spectra automatically when you click t

Table of Contents About This Manual ... iii

3: File Menu Functions Configure The File | Autoincrement Filenames | Configure option displays a dialog with the following parameters: Base Name Se

Chapter 4 Edit Menu Functions Overview This section details the various options and functions available from the Edit menu in OOIBase32. Where applic

4: Edit Menu Functions Click on the Copy Spectra icon or select Edit | Copy Graphical Spectra to copy graphical spectra to the Windows clipboard. S

4: Edit Menu Functions File Saving This tab provides options for saving files. Available options include the following: Option Function Always Sav

4: Edit Menu Functions Option Function Saved Precision Select a value from 0 to 10 decimal places to specify the precision of the spectral data. Sav

4: Edit Menu Functions Printing This tab specifies whether the graph window prints in black and white or color. Select the Printing in Black and Whi

4: Edit Menu Functions Available options include the following: Option Function Enable Sounds Enables sound events in OOIBase32 Filename This text

4: Edit Menu Functions Available options include the following: Option Function Default Display Settings File Contains settings such as trace color

4: Edit Menu Functions Registration This tab allows you to enter an Operator name and the software Serial Number. OOIBase32 includes these entries i

4: Edit Menu Functions Available options include the following: Option Function Warn Before Overlay Spectrum is Cleared Enable this setting to recei

Table of Contents Open...

4: Edit Menu Functions Option Function Percent Transmission Mode Label Select the axis label to reflect the type of Transmission mode measurement y

Chapter 5 View Menu Functions Overview This chapter details the various options and functions available from the View menu in OOIBase32. Where app

5: View Menu Functions Active Line Type These settings allow you to set the design of the line connecting the data points in an active spectrum. Th

5: View Menu Functions Click on each drop-down menu or color box to set these options: Option Function Point Style Sets the style (circle, square,

5: View Menu Functions Click on each drop-down menu or color box to set these options: Option Function Line Style Sets the style. Line Width Sets t

5: View Menu Functions Click on each drop-down menu or color box to set these options: Option Function Point Style Sets the style (circle, square,

5: View Menu Functions Restore Display Settings This option retrieves the previously saved display settings from the disk. Select View | Display Pr

5: View Menu Functions Autoscale - Graph Scale Toolbar This option adjusts the scale of the spectrum displayed in the current spectral window so

5: View Menu Functions Set Scale - Graph Scale Toolbar This option allows you to specify the minimum and maximum limits of both the wavelength an

5: View Menu Functions Select Bitmap This option allows you to choose a background picture to display as a background image in the graph window of O

Table of Contents Chapter 5: View Menu Functions...31Overview...

5: View Menu Functions Set Axis Text Properties This option allows you to choose a color for text in the spectral window. Select View | Set Axis Tex

5: View Menu Functions Cursor These options enable you to specify cursor display properties in OOIBase32. Enabled - Cursor Controls Toolbar This op

5: View Menu Functions Option Function Limit Cursor to Displayed Wavelength Range Restricts the cursor to the wavelengths displayed in the graph win

5: View Menu Functions Legend These options allow you to configure the appearance of the legend in the graph window of OOIBase32. 000-20000-020-02

5: View Menu Functions Enabled This option enables or disables the display of the legend in the spectral window. Select View | Legend | Enabled. Con

5: View Menu Functions Show Overlay Filename This option enables or disables the display of the overlay filename (when you specify an overlay) in th

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Chapter 6 Overlay Menu Functions Overview This section details the various options and functions available from the Overlay menu in OOIBase32. Where

6: Overlay Menu Functions Note To configure OOIBase32 to present a warning before clearing an overlay, select Edit | Settings from the menu and choo

Chapter 7 Spectrometer Menu Functions Overview This section details the various options and functions available from the Spectrometer menu in OOIBa

Table of Contents Chapter 7: ...49Spectrometer Menu FunctionsOverview...

7: Spectrometer Menu Functions When using a USB-based spectrometer or A/D interface, the coefficients for the spectrometer automatically load as par

7: Spectrometer Menu Functions A/D Interface Tab The A/D Interface tab of the Spectrometer Configuration dialog box allows you to set the hardware p

7: Spectrometer Menu Functions Option Description SAD500 Baud Rate Specifies the communication speed at which the SAD500 A/D converter operates. Thi

7: Spectrometer Menu Functions Note If your reference is in a region that has low scope mode intensity, you will introduce noise into the corrected

7: Spectrometer Menu Functions Software Configuration: 1. Open OOIBase32 and select Spectrometer | Configure. 2. Select the Reference Monitoring t

7: Spectrometer Menu Functions ► Procedure Perform the steps below to monitor a reference using the Integrated Intensity option: Hardware Configura

7: Spectrometer Menu Functions • You cannot afford to sample a wavelength area and must monitor a non-absorbing wavelength region ► Procedure Perfo

7: Spectrometer Menu Functions The table below details the options available in the Stray Light Correction tab. Option Description Spectrometer Cha

7: Spectrometer Menu Functions The software automatically populates the values in the Detector Linearity tab from the information on the spectromete

7: Spectrometer Menu Functions Save Configuration As This option allows you to save the current spectrometer configuration parameters. After you nam

Table of Contents Reference Color Temperature... 72 Configure

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Chapter 8 Spectrum Menu Functions Overview This section details the various options and functions available from the Spectrum menu in OOIBase32. Wh

8: Spectrum Menu Functions Click on the Store Reference icon ( ) or select Spectrum | Store Reference from the menu. Snapshot - Spectrum Controls T

8: Spectrum Menu Functions Global Global functions apply to all spectral windows in OOIBase32. Store Global Dark - Global Functions Toolbar This op

8: Spectrum Menu Functions command sets the integration time to 100 msec, acquires one average, and turns off spectral smoothing and external trigge

8: Spectrum Menu Functions This tab contains the following options: Option Function Integration Time (msec) Specifies the integration time (or A/D c

8: Spectrum Menu Functions External Trigger Tab The External Trigger tab contains options that allow you to configure your sampling system to acquir

8: Spectrum Menu Functions This tab contains the following options: Option Description Strobe Enable Enables or disables the spectrometer strobe co

8: Spectrum Menu Functions Available options on the HR4000/HR2000+ Features dialog box include the following: Option Description Single Strobe Timi

8: Spectrum Menu Functions Option Description External Trigger This setting sets the delay between the assertion of an external trigger and the st

Table of Contents Application Tips ...

8: Spectrum Menu Functions Scope Mode Minus Dark - Spectral View Mode Toolbar This command switches the current spectral window into Scope mode, an

8: Spectrum Menu Functions Transmission Mode - Spectral View Mode Toolbar This command switches the current window into Transmission mode. This is

8: Spectrum Menu Functions Specular Reflection Mode - Spectral View Mode Toolbar This command switches the current window into Reflection mode. Thi

8: Spectrum Menu Functions You must enter the color temperature to use Relative Irradiance mode. Select Spectrum | Reference Color Temperature. Con

8: Spectrum Menu Functions Store Reference Dark This option stores a reference dark spectrum for all enabled spectrometer channels in the active spe

Chapter 9 Time Acquisition Menu Functions Overview This section details the various options and functions available from the Time Acquisition menu

9: Time Acquisition Menu Functions Available options on the Time Acquisition Configuration dialog box include the following: Option Description Str

10: Other Menu Functions Option Description Initial Delay Determines the initial delay for a time acquisition. OOIBase32 introduces this delay a

9: Time Acquisition Menu Functions Time Channels A Through F These tabs allow you to configure a time acquisition process for a single wavelength. S

10: Other Menu Functions Time Channels Combination 1 and 2 These tabs allow you to configure a time acquisition process for a combination of two t

About This Manual Document Purpose and Intended Audience This document provides you with installation and configuration instructions section to get

9: Time Acquisition Menu Functions Restore Parameters This option opens a dialog box that prompts you to navigate to and open a file with the comple

10: Other Menu Functions Pause - Time Acquisition Toolbar This option pauses the time acquisition process. OOIBase32 enables this option once you

9: Time Acquisition Menu Functions OOIBase32 stores the data in a tab-delimited ASCII file, with time data arranged in columns. The first column is

Chapter 10 Other Menu Functions Overview This section details the various options and functions available from other menus in OOIBase32 not previous

10: Other Menu Functions Arrange Icons This command arranges all iconic windows. Select Window | Arrange Icons. Help Menu Functions The Help Menu co

10: Other Menu Functions About OOIBase32 This command displays the About OOIBase32 dialog box. This dialog box contains the following information

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Appendix A Experiment Tutorials Overview The following sections contain information on conducting sample experiments using the USB2000 Spectrometer

A: Experiment Tutorials Once you install the hardware, configure the software, and establish your sampling system, you are ready to take measurement

A: Experiment Tutorials Absorbance Experiments Absorbance spectra are a measure of how much light a sample absorbs. For most samples, absorbance